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NAD+ (500mg & 1000mg) (500mg Vial)
1 × $86.00
Subtotal: $86.00
NAD+ (500mg & 1000mg) (1000mg Vial)
$140.00
Nicotinamide adenine dinucleotide — the dinucleotide coenzyme central to redox metabolism — supplied lyophilized for in-vitro biochemistry research.
In stock
Tested by Freedom Diagnostics TestingHPLC purity + LC-MS identityLot-matched COA on-site
For research use only. This product is a laboratory research chemical. It is not for human or veterinary use, not a drug, supplement, or food, and not intended to diagnose, treat, cure, or prevent any disease. Sold only to qualified researchers and institutions.
What is NAD+?
NAD+ (β-nicotinamide adenine dinucleotide) is a dinucleotide coenzyme — not a peptide — that sits at the center of cellular redox metabolism. Built from a nicotinamide nucleotide joined to an adenine nucleotide, it cycles between its oxidized form (NAD+) and reduced form (NADH) to shuttle electrons between metabolic reactions. In this role it is the universal electron acceptor for the dehydrogenase enzymes of glycolysis, the citric-acid cycle, β-oxidation, and oxidative phosphorylation.
Beyond redox chemistry, NAD+ is also consumed as a substrate by a family of NAD+-cleaving enzymes — the sirtuins (SIRT1–SIRT7), the poly(ADP-ribose) polymerases (PARP1, PARP2), and the glycohydrolases CD38 and CD157. Research areas where laboratories buy NAD+ for in vitro work include dehydrogenase enzyme kinetics read via the 340 nm NAD+/NADH absorbance shift, sirtuin and PARP activity assays, cell-culture redox studies measuring the NAD+/NADH ratio, mitochondrial bioenergetics, and comparative studies of NAD+ replenishment versus precursor supplementation (NMN, NR, niacin).
Every lot, independently verified
Independently third-party tested and matched to the lot ID printed on your vial. Values below are from the latest released lot.
99.95%
HPLC-UV Purity
Passed full QC panel
independent third-party lab (Freedom Diagnostics Testing) accredited lab
Latest batch · tested 23 Dec 2025 · Freedom Diagnostics (USA)
Lot details
ProductNAD+ 1000 mg
Lot25092401
AppearanceWhite lyophilized powder
Accession #2512220125
Reported23 Dec 2025
Full QC panel · ISO 170257 assays
Purity (HPLC-UV)99.952% ✓
Identity (LC-MS)NAD+ ✓
HPLC conformity (2×)Confirmed ✓
Net content888.08 mg ✓
Heavy metals (ICP-MS)Not Detected ✓
SterilityNo Growth ✓
Endotoxin (USP <85>)Within limits ✓
Every lot is released against Koi’s full QC panel — HPLC purity, LC-MS identity, net content, heavy metals, sterility, and endotoxin — by an independent ISO/IEC 17025-accredited laboratory. Values shown are from the latest released lot (Lot 25092401, reported 23 Dec 2025) and vary batch to batch; the lot-specific certificate is published in our public COA library and matched to the lot ID printed on the vial.
Compound information
Chemical & structural reference data — specifications, molecular profile and handling.
Product Specifications
CompoundNAD+ (β-nicotinamide adenine dinucleotide, dinucleotide coenzyme)
Vial size500 mg & 1000 mg
Purity≥99% by HPLC
Identity confirmationMass spectrometry
FormWhite to off-white powder
Appearance after reconstitutionClear, colorless solution
ManufacturingManufactured and lyophilized in the United States
Quality testingIndependent independent third-party laboratory release
Molecular Profile
Chemical nameβ-Nicotinamide adenine dinucleotide
Molecular formulaC21H27N7O14P2
Molecular weight663.43 g/mol
CAS number53-84-9
PubChem CID925
Other namesβ-NAD+ · Coenzyme I · DPN+
Structure classDinucleotide coenzyme · free acid
Storage & Handling
AppearanceWhite to off-white powder
SolubilityWater / PBS / Tris · neutral pH
Storage · powder−20 °C, desiccated, dark (−80 °C long-term)
In solutionUse same day; avoid alkaline buffers
NAD+ Mechanism of Action
- Accepts a hydride (H−) at the C4 position of the nicotinamide ring to convert NAD+ to NADH — the central two-electron transfer step in cellular redox chemistry.
- Acts as the cofactor for dehydrogenase enzymes in glycolysis, the citric-acid cycle, β-oxidation, and oxidative phosphorylation.
- Functions as a substrate for sirtuins (SIRT1–SIRT7), coupling deacylation of histone and protein lysines to NAD+ cleavage and nicotinamide release.
- Serves as the ADP-ribose donor for PARP1 and PARP2 in poly(ADP-ribose) polymerase reactions on damaged DNA strands.
- Provides the substrate for CD38, CD157, and SARM1 — NAD+-cleaving enzymes in calcium signaling, immune activity, and axon-degeneration pathways.
- Sits at the head of a salvage pathway in which nicotinamide is recycled back into NAD+ via NMN through the NAMPT/NMNAT cascade.
NAD+ Research Applications
- Dehydrogenase enzyme kinetics using NAD+ as the electron acceptor, read directly via the 340 nm absorbance shift between NAD+ and NADH.
- Sirtuin (SIRT1–SIRT7) activity assays, where NAD+ is the co-substrate and nicotinamide release is quantified.
- PARP enzyme activity profiling in DNA-damage-response models, with NAD+ as the ADP-ribose donor.
- Cell-culture redox studies measuring the NAD+/NADH ratio across metabolic states using biosensors or mass spectrometry.
- Mitochondrial bioenergetics — how NAD+ availability influences electron-transport-chain flux, oxygen consumption, and ATP synthesis.
- Comparative studies of NAD+ replenishment versus precursor supplementation (NMN, NR, niacin) across cell types.
Why Buy NAD+ from Koi Peptides?
- Every batch of Koi NAD+ is manufactured and lyophilized in the United States, then released against independent third-party laboratory (Freedom Diagnostics Testing) testing.
- Each lot is tested by HPLC for purity, LC-MS for identity confirmation, and endotoxin assay for sterility readiness.
- The lot ID is printed on every vial and ties back to a public COA library, so researchers can verify documentation before they buy and after the vial arrives.
- Orders placed before 2 PM CT ship the same business day, and shipping is free on orders over $200.
- Belenky, P., et al. (2007). NAD+ metabolism in health and disease. Trends in Biochemical Sciences, 32(1), 12–19. doi.org/10.1016/j.tibs.2006.11.006
- Bernofsky, C., & Swan, M. (1973). An improved cycling assay for nicotinamide adenine dinucleotide. Analytical Biochemistry, 53(2), 452–458. doi.org/10.1016/0003-2697(73)90094-8
- Imai, S., & Guarente, L. (2014). NAD+ and sirtuins in aging and disease. Trends in Cell Biology, 24(8), 464–471. doi.org/10.1016/j.tcb.2014.04.002
- Bai, P. (2015). Biology of poly(ADP-ribose) polymerases. Molecular Cell, 58(6), 947–958. doi.org/10.1016/j.molcel.2015.01.034
- Verdin, E. (2015). NAD+ in aging, metabolism, and neurodegeneration. Science, 350(6265), 1208–1213. doi.org/10.1126/science.aac4854
- Covarrubias, A. J., et al. (2021). NAD+ metabolism and its roles in cellular processes during ageing. Nature Rev. Mol. Cell Biol., 22(2), 119–141. doi.org/10.1038/s41580-020-00313-x
- Chini, C. C. S., et al. (2020). CD38 ecto-enzyme regulates NAD+ and NMN levels in aging. Nature Metabolism, 2(11), 1284–1304. doi.org/10.1038/s42255-020-00298-z
- Cambronne, X. A., et al. (2016). Biosensor reveals multiple sources for mitochondrial NAD+. Science, 352(6292), 1474–1477. doi.org/10.1126/science.aad5168
- Yoshino, J., et al. (2018). NAD+ intermediates: the biology & therapeutic potential of NMN and NR. Cell Metabolism, 27(3), 513–528. doi.org/10.1016/j.cmet.2017.11.002
Regulatory Status
FDA, WADA anti-doping, and compounding status — provided for research context.
FDA StatusNAD+ is a naturally occurring coenzyme that is marketed as a dietary-supplement ingredient in some contexts, but it is not an FDA-approved drug for any indication. The research-grade material supplied by Koi Peptides is provided for in vitro laboratory research only — not formulated, packaged, or labeled for human use, not a dietary supplement product, and not authorized for any human or veterinary indication.
WADA Anti-Doping StatusNot prohibited. NAD+ is not named on the WADA Prohibited List. As with all compounds, athletes and researchers should consult the current WADA List directly, since classifications are reviewed annually. This information is provided for research context only and does not constitute compliance advice.
Compounding Pharmacy StatusKoi Peptides is a chemical research supplier — not a compounding pharmacy or outsourcing facility under sections 503A or 503B of the Federal Food, Drug, and Cosmetic Act — and supplies no compounded preparations of any kind. The research-grade NAD+ supplied here is not pharmaceutical-grade material intended for sterile compounding for human use.
Disclaimer
For research use only. Not for human or veterinary use. NAD+ is supplied to qualified research professionals for in vitro laboratory study. It has not been evaluated by the FDA and is not intended to diagnose, treat, cure, or prevent any disease. Koi Research Labs LLC is a chemical supplier, not a compounding pharmacy under 503A nor an outsourcing facility under 503B of the Federal Food, Drug, and Cosmetic Act. By purchasing, the buyer agrees this material will not be introduced into humans or animals. Misuse may violate federal, state, or local laws.
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